Journal: The Journal of biological chemistry
Article Title: Rapid genomic changes by mineralotropic hormones and kinase SIK inhibition drive coordinated renal Cyp27b1 and Cyp24a1 expression via CREB modules.
doi: 10.1016/j.jbc.2022.102559
Figure Lengend Snippet: Figure 10. Transcriptional implications of treatments at Cyp27b1. ChIP-Seq analysis near Cyp27b1 for (A) BRD4, (B) RNA polymerase II, and (C) H3K36me3 from WT mice injected with 230 mg/kg bw PTH (30 min, PTH30), 30 mg/kg bw YKL-05 to 099 (1 h), 40 mg/kg bw SK-124 (1 h), 10 mg/kg bw 1,25(OH)2D3 (1 h), or 50 mg/kg bw FGF23 (1 h). Additional details as for Figure 2. Genomic region displayed is chr10: 126,469,260 to 126,490,800. bw, body weight; ChIP- Seq, chromatin immunoprecipitation; FGF23, fibroblast growth factor 23; 1,25(OH)2D3, 1,25-dihydroxyvitamin D3; PTH, parathyroid hormone.
Article Snippet: The following reagents were used for in vivo injections: 1α,25(OH)2D3 was obtained from SAFC Global, PTH (1–84 human) was obtained from Bachem (H-1370.0100), mouse FGF23 from R&D Systems (2629-FG-025), YKL-05-099 (27), and SK-124 (26).
Techniques: ChIP-sequencing, Injection, Chromatin Immunoprecipitation